The purposes of this project were to undertake the research of susceptibility with 2015-formed insecticide discrimination dose or diagnostic dose (concentration) and to test the effect of public health pesticides. The test was conducted among traditional markets in Kaohsiung area to examine on the susceptibility of roaches of 5 field strains (Daliao district, Siaogang district, Gushan district, Gangshan and Sanmin district). The result showed that Blattella germanica (Siaogang district and Gangshan district strains) in Kaohsiung area and Periplaneta americana (Daliao district and Sanmin district strains) were resistant to permethrin. B. germanica (Gangshan district strain) and P. americana (Gushan district strain) were resistant to deltamethrin. B. germanica were resistant to both permethrin and deltamethrin , also showed cross-resistance. Musca domestica of 3 field strains in Kaohsiung area ( Gangshan district, Dashe district and Fongshan district strains) were all resistant to cypermethrin, permethrin and deltamethrin, and showed the cross-resistance. Chrysomyia megacephala (Dashe district strain) was resistant to chlopyrifos; Drosophila melanogaster of 3 field strains in Kaohsiung area all showed no resistance; Cimex lectularius of 3 field strains ( Tzukuan district, Kaohsiung, Fongshan district, Kaohsiung and North district, Tainan) in Taiwan all showed no resistance. According to the test results, the residual effects of public health pesticides, 8 emulsions and 4 aerosols, on B. germanica and P. americana showed the mortality rate within 24 hours was 100%, the mortality rate within 24 hours on seventh day and fourteenth day were all greater than 70%. The results were conformed with the examination guidelines on the effect of public health pesticides of Environmental Protection Administration, Executive Yuan, R.O.C. (The mortality rate within 24 hours of residual effect is greater than 70%.) By using glass-tube method, contact with films of pesticide method and peet grady chamber method, the effects of public health pesticides, 5 liquors, 3 lacquers and 2 aerosols, on Musca domestica, Chrysomyia megacephala and Drosophila melanogaster all showed the mortality rate within 24 hours was 100%. In addition, the KT50 of 3 lacquers and 2 aerosols were all shorter than 8 minutes. The results were conformed with flies’ examination guidelines on the effect of public health pesticides of Environmental Protection Administration, Executive Yuan, R.O.C. (KT50 which is shorter than 8 minutes shows the stunning effect and the mortality rate within 24 hours is greater than 80 %.) By using the contact with glass-petri dish method, the effects of public health pesticides, 5 liquors and 2 aerosols, on Cimex lectularius all showed the mortality rate within 24 hours was 100%, however, among the public health pesticides, the residual effects of 5 liquors and 1 aerosol on C. lectularius showed the mortality rate within 24 hours on third day, fifth day and seventh day were all less than 70%. The results were failed to the residual effect of examination guidelines. (The approved residual effect shows the mortality rate within 24 hours is greater than 70 %.) To sum up, filing susceptibility data of Environmental pest strains can provide effective scientific references for pest controlling services, public health pesticides manufacturers, environmental protection agencies and the public.

insecticide, discriminating dose or diagnostic dose, resistant, bioassay